DDX3X report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0032508Biological processDNA duplex unwinding
GO_1901224Biological processpositive regulation of non-canonical NF-kappaB signal transduction
GO_0032728Biological processpositive regulation of interferon-beta production
GO_1902523Biological processpositive regulation of protein K63-linked ubiquitination
GO_0031333Biological processnegative regulation of protein-containing complex assembly
GO_1900087Biological processpositive regulation of G1/S transition of mitotic cell cycle
GO_0043066Biological processnegative regulation of apoptotic process
GO_0042256Biological processcytosolic ribosome assembly
GO_1902042Biological processnegative regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO_0055088Biological processlipid homeostasis
GO_2001243Biological processnegative regulation of intrinsic apoptotic signaling pathway
GO_0010501Biological processRNA secondary structure unwinding
GO_0071243Biological processcellular response to arsenic-containing substance
GO_0071651Biological processpositive regulation of chemokine (C-C motif) ligand 5 production
GO_0034161Biological processpositive regulation of toll-like receptor 8 signaling pathway
GO_0034063Biological processstress granule assembly
GO_0010629Biological processnegative regulation of gene expression
GO_0045087Biological processinnate immune response
GO_0010628Biological processpositive regulation of gene expression
GO_0034157Biological processpositive regulation of toll-like receptor 7 signaling pathway
GO_0097193Biological processintrinsic apoptotic signaling pathway
GO_0071470Biological processcellular response to osmotic stress
GO_0045727Biological processpositive regulation of translation
GO_0030308Biological processnegative regulation of cell growth
GO_0043154Biological processnegative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO_0036493Biological processpositive regulation of translation in response to endoplasmic reticulum stress
GO_0007059Biological processchromosome segregation
GO_0090263Biological processpositive regulation of canonical Wnt signaling pathway
GO_0008625Biological processextrinsic apoptotic signaling pathway via death domain receptors
GO_0006413Biological processtranslational initiation
GO_0032481Biological processpositive regulation of type I interferon production
GO_0032727Biological processpositive regulation of interferon-alpha production
GO_0045070Biological processpositive regulation of viral genome replication
GO_1901223Biological processnegative regulation of non-canonical NF-kappaB signal transduction
GO_0016055Biological processWnt signaling pathway
GO_0043065Biological processpositive regulation of apoptotic process
GO_0017148Biological processnegative regulation of translation
GO_0045944Biological processpositive regulation of transcription by RNA polymerase II
GO_1901985Biological processpositive regulation of protein acetylation
GO_0045948Biological processpositive regulation of translational initiation
GO_0030307Biological processpositive regulation of cell growth
GO_1903608Biological processprotein localization to cytoplasmic stress granule
GO_0009615Biological processresponse to virus
GO_0007276Biological processgamete generation
GO_0043280Biological processpositive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO_1900227Biological processpositive regulation of NLRP3 inflammasome complex assembly
GO_0098586Biological processcellular response to virus
GO_0035556Biological processintracellular signal transduction
GO_0030154Biological processcell differentiation
GO_0031954Biological processpositive regulation of protein autophosphorylation
GO_0071902Biological processpositive regulation of protein serine/threonine kinase activity
GO_0072559Cellular componentNLRP3 inflammasome complex
GO_1904813Cellular componentficolin-1-rich granule lumen
GO_0010494Cellular componentcytoplasmic stress granule
GO_0005634Cellular componentnucleus
GO_0005576Cellular componentextracellular region
GO_0070062Cellular componentextracellular exosome
GO_0005654Cellular componentnucleoplasm
GO_0030027Cellular componentlamellipodium
GO_0005886Cellular componentplasma membrane
GO_0005813Cellular componentcentrosome
GO_0034774Cellular componentsecretory granule lumen
GO_0031252Cellular componentcell leading edge
GO_0005737Cellular componentcytoplasm
GO_0005852Cellular componenteukaryotic translation initiation factor 3 complex
GO_0043186Cellular componentP granule
GO_0022627Cellular componentcytosolic small ribosomal subunit
GO_0005829Cellular componentcytosol
GO_0008134Molecular functiontranscription factor binding
GO_0043015Molecular functiongamma-tubulin binding
GO_0043539Molecular functionprotein serine/threonine kinase activator activity
GO_0005524Molecular functionATP binding
GO_0031369Molecular functiontranslation initiation factor binding
GO_0003678Molecular functionDNA helicase activity
GO_0003924Molecular functionGTPase activity
GO_0003724Molecular functionRNA helicase activity
GO_0017111Molecular functionribonucleoside triphosphate phosphatase activity
GO_0043273Molecular functionCTPase activity
GO_0003729Molecular functionmRNA binding
GO_0035591Molecular functionsignaling adaptor activity
GO_0003723Molecular functionRNA binding
GO_0005515Molecular functionprotein binding
GO_0003677Molecular functionDNA binding
GO_0008143Molecular functionpoly(A) binding
GO_0048027Molecular functionmRNA 5'-UTR binding
GO_0045296Molecular functioncadherin binding
GO_0043024Molecular functionribosomal small subunit binding
GO_0033592Molecular functionRNA strand annealing activity
GO_0008190Molecular functioneukaryotic initiation factor 4E binding
GO_0035613Molecular functionRNA stem-loop binding
GO_0016887Molecular functionATP hydrolysis activity

IV. Literature review

[source]
Gene nameDDX3X
Protein nameDEAD-box helicase 3 X-linked
ATP-dependent RNA helicase DDX3X (EC 3.6.4.13) (CAP-Rf) (DEAD box protein 3, X-chromosomal) (DEAD box, X isoform) (DBX) (Helicase-like protein 2) (HLP2)
RNA helicase (EC 3.6.4.13)
SynonymsDDX3
DBX
DescriptionFUNCTION: Multifunctional ATP-dependent RNA helicase . The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity . In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs . Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA . Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities (Probable). Involved in transcription regulation . Positively regulates CDKN1A/WAF1/CIP1 transcription in an SP1-dependent manner, hence inhibits cell growth. This function requires its ATPase, but not helicase activity . CDKN1A up-regulation may be cell-type specific . Binds CDH1/E-cadherin promoter and represses its transcription . Potentiates HNF4A-mediated MTTP transcriptional activation; this function requires ATPase, but not helicase activity. Facilitates HNF4A acetylation, possibly catalyzed by CREBBP/EP300, thereby increasing the DNA-binding affinity of HNF4 to its response element. In addition, disrupts the interaction between HNF4 and SHP that forms inactive heterodimers and enhances the formation of active HNF4 homodimers. By promoting HNF4A-induced MTTP expression, may play a role in lipid homeostasis . May positively regulate TP53 transcription . Associates with mRNPs, predominantly with spliced mRNAs carrying an exon junction complex (EJC) . Involved in the regulation of translation initiation . Not involved in the general process of translation, but promotes efficient translation of selected complex mRNAs, containing highly structured 5'-untranslated regions (UTR) . This function depends on helicase activity . Might facilitate translation by resolving secondary structures of 5'-UTRs during ribosome scanning . Alternatively, may act prior to 43S ribosomal scanning and promote 43S pre-initiation complex entry to mRNAs exhibiting specific RNA motifs, by performing local remodeling of transcript structures located close to the cap moiety . Independently of its ATPase activity, promotes the assembly of functional 80S ribosomes and disassembles from ribosomes prior to the translation elongation process . Positively regulates the translation of cyclin E1/CCNE1 mRNA and consequently promotes G1/S-phase transition during the cell cycle . May activate TP53 translation . Required for endoplasmic reticulum stress-induced ATF4 mRNA translation . Independently of its ATPase/helicase activity, enhances IRES-mediated translation; this activity requires interaction with EIF4E . Independently of its ATPase/helicase activity, has also been shown specifically repress cap-dependent translation, possibly by acting on translation initiation factor EIF4E . Involved in innate immunity, acting as a viral RNA sensor. Binds viral RNAs and promotes the production of type I interferon (IFN-alpha and IFN-beta) . Potentiate MAVS/RIGI-mediated induction of IFNB in early stages of infection . Enhances IFNB1 expression via IRF3/IRF7 pathway and participates in NFKB activation in the presence of MAVS and TBK1 . Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFNB induction, acts as a scaffolding adapter that links IKBKE and IRF3 and coordinates their activation . Involved in the TLR7/TLR8 signaling pathway leading to type I interferon induction, including IFNA4 production. In this context, acts as an upstream regulator of IRF7 activation by MAP3K14/NIK and CHUK/IKKA. Stimulates CHUK autophosphorylation and activation following physiological activation of the TLR7 and TLR8 pathways, leading to MAP3K14/CHUK-mediated activatory phosphorylation of IRF7 . Also stimulates MAP3K14/CHUK-dependent NF-kappa-B signaling . Negatively regulates TNF-induced IL6 and IL8 expression, via the NF-kappa-B pathway. May act by interacting with RELA/p65 and trapping it in the cytoplasm . May also bind IFNB promoter; the function is independent of IRF3 . Involved in both stress and inflammatory responses (By similarity). Independently of its ATPase/helicase activity, required for efficient stress granule assembly through its interaction with EIF4E, hence promotes survival in stressed cells . Independently of its helicase activity, regulates NLRP3 inflammasome assembly through interaction with NLRP3 and hence promotes cell death by pyroptosis during inflammation. This function is independent of helicase activity (By similarity). Therefore DDX3X availability may be used to interpret stress signals and choose between pro-survival stress granules and pyroptotic NLRP3 inflammasomes and serve as a live-or-die checkpoint in stressed cells (By similarity). In association with GSK3A/B, negatively regulates extrinsic apoptotic signaling pathway via death domain receptors, including TNFRSF10B, slowing down the rate of CASP3 activation following death receptor stimulation . Cleavage by caspases may inactivate DDX3X and relieve the inhibition . Independently of its ATPase/helicase activity, allosteric activator of CSNK1E. Stimulates CSNK1E-mediated phosphorylation of DVL2, thereby involved in the positive regulation of Wnt/beta-catenin signaling pathway. Also activates CSNK1A1 and CSNK1D in vitro, but it is uncertain if these targets are physiologically relevant . ATPase and casein kinase-activating functions are mutually exclusive . May be involved in mitotic chromosome segregation . .; FUNCTION: (Microbial infection) Facilitates hepatitis C virus (HCV) replication . During infection, HCV core protein inhibits the interaction between MAVS and DDX3X and therefore impairs MAVS-dependent INFB induction and might recruit DDX3X to HCV replication complex . .; FUNCTION: (Microbial infection) Facilitates HIV-1 replication . Acts as a cofactor for XPO1-mediated nuclear export of HIV-1 Rev RNAs . This function is strongly stimulated in the presence of TBK1 and requires DDX3X ATPase activity . .; FUNCTION: (Microbial infection) Facilitates Zika virus (ZIKV) replication. .; FUNCTION: (Microbial infection) Facilitates Dengue virus (DENV) replication. .; FUNCTION: (Microbial infection) Facilitates Venezuelan equine encephalitis virus (VEEV) replication. .

AccessionsENST00000644677.1
A0A2R8YGJ1
ENST00000616050.3
A0A2R8Y4A4
A0A0J9YVQ7
A0A2R8Y774
ENST00000615742.4
ENST00000644513.1
F6S8Q4
ENST00000615313.5
A0A2R8Y7T2
A0A2U3TZJ9
ENST00000625837.2
ENST00000642793.1
ENST00000646627.1
A0A0D9SFB3
ENST00000644876.2 [O00571-1]
ENST00000644074.1
A0A0D9SF53
ENST00000630858.2
ENST00000645589.1
ENST00000626301.2
ENST00000647373.1
ENST00000645783.1
ENST00000611968.2
ENST00000629785.2 [O00571-1]
ENST00000646223.1
A0A087WVZ1
ENST00000646107.1
A0A2R8Y5G6
B5BTY4
A0A2R8Y645
A0A2R8YFS5
ENST00000642322.2
A0A2R8YF31
ENST00000399959.7
ENST00000643821.1
A0A2R8YDT5
ENST00000643963.1
ENST00000478993.5 [O00571-1]
ENST00000646319.1
ENST00000457138.7 [O00571-2]
ENST00000629496.3 [O00571-1]
A0A2R8YF78
A0A0D9SG12
ENST00000630370.2
ENST00000630255.2 [O00571-1]
A0A2R8Y4D2
A0A2R8YDH3
A0A087WX09
ENST00000644109.1
A0A2R8Y650
ENST00000646122.1
A0A2R8YCU0
ENST00000644073.1
O00571
ENST00000441189.4
ENST00000646679.1
ENST00000645080.1
ENST00000642424.1
A0A2R8YFR4
A0A2R8YCW1
A0A2R8YF89
ENST00000542215.5
A0A2R8Y770