NEMF report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0051168Biological processnuclear export
GO_1990116Biological processribosome-associated ubiquitin-dependent protein catabolic process
GO_0072344Biological processrescue of stalled ribosome
GO_0140708Biological processCAT tailing
GO_0065003Biological processprotein-containing complex assembly
GO_1990112Cellular componentRQC complex
GO_0022626Cellular componentcytosolic ribosome
GO_0005829Cellular componentcytosol
GO_0005634Cellular componentnucleus
GO_0043023Molecular functionribosomal large subunit binding
GO_1904678Molecular functionalpha-aminoacyl-tRNA binding
GO_0000049Molecular functiontRNA binding

IV. Literature review

[source]
Gene nameNEMF
Protein nameRibosome quality control complex subunit NEMF (Antigen NY-CO-1) (Nuclear export mediator factor) (Serologically defined colon cancer antigen 1)
Nuclear export mediator factor
Alternative protein NEMF
SynonymsSDCCAG1
DescriptionFUNCTION: Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation . Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell . Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits . Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A-site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal alanine extensions (CAT tails) . Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs . CAT tailing is required to promote ubiquitination of stalled nascent chains by different E3 ubiquitin-protein ligases . In the canonical RQC pathway (RQC-L), CAT tailing facilitates LTN1-dependent ubiquitination by exposing lysine residues that would otherwise remain buried in the ribosomal exit tunnel (By similarity). In the alternative RQC pathway (RQC-C) CAT tailing creates an C-degron mainly composed of alanine that is recognized by the CRL2(KLHDC10) and RCHY1/PIRH2 E3 ligases, leading to ubiquitination and degradation of stalled nascent chains . NEMF may also indirectly play a role in nuclear export . .

AccessionsENST00000298310.10 [O60524-1]
L8E842
O60524
ENST00000554626.5
ENST00000556672.1
G3V5V3
ENST00000555970.5
L8E7P7
G3V547
G3V4D9