CARD8 report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0006919Biological processactivation of cysteine-type endopeptidase activity involved in apoptotic process
GO_1900226Biological processnegative regulation of NLRP3 inflammasome complex assembly
GO_0051607Biological processdefense response to virus
GO_0032691Biological processnegative regulation of interleukin-1 beta production
GO_0043280Biological processpositive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO_0032088Biological processnegative regulation of NF-kappaB transcription factor activity
GO_0032731Biological processpositive regulation of interleukin-1 beta production
GO_0042981Biological processregulation of apoptotic process
GO_0097264Biological processself proteolysis
GO_0031665Biological processnegative regulation of lipopolysaccharide-mediated signaling pathway
GO_0140633Biological processCARD8 inflammasome complex assembly
GO_0006915Biological processapoptotic process
GO_0097340Biological processinhibition of cysteine-type endopeptidase activity
GO_0043122Biological processregulation of canonical NF-kappaB signal transduction
GO_0010804Biological processnegative regulation of tumor necrosis factor-mediated signaling pathway
GO_0043124Biological processnegative regulation of canonical NF-kappaB signal transduction
GO_0140374Biological processantiviral innate immune response
GO_0072559Cellular componentNLRP3 inflammasome complex
GO_0032991Cellular componentprotein-containing complex
GO_0005654Cellular componentnucleoplasm
GO_0005829Cellular componentcytosol
GO_0005737Cellular componentcytoplasm
GO_0140634Cellular componentCARD8 inflammasome complex
GO_0005634Cellular componentnucleus
GO_0050700Molecular functionCARD domain binding
GO_0042803Molecular functionprotein homodimerization activity
GO_0008233Molecular functionpeptidase activity
GO_0038187Molecular functionpattern recognition receptor activity
GO_0008656Molecular functioncysteine-type endopeptidase activator activity involved in apoptotic process
GO_0032089Molecular functionNACHT domain binding
GO_0140693Molecular functionmolecular condensate scaffold activity
GO_0140608Molecular functioncysteine-type endopeptidase activator activity
GO_0005515Molecular functionprotein binding

IV. Literature review

[source]
Gene nameCARD8
Protein nameCaspase recruitment domain family member 8
CARD8 protein
CARD8 isoform T51
Caspase recruitment domain-containing protein 8 (EC 3.4.-.-) (CARD-inhibitor of NF-kappa-B-activating ligand) (CARDINAL) (Tumor up-regulated CARD-containing antagonist of CASP9) (TUCAN) [Cleaved into: Caspase recruitment domain-containing protein 8, C-terminus (CARD8-CT); Caspase recruitment domain-containing protein 8, N-terminus (CARD8-NT)]
SynonymsNDPP1
KIAA0955
DACAR
DescriptionFUNCTION: Inflammasome sensor, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis of CD4(+) T-cells and macrophages . Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation . Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as HIV-1 protease activity or Val-boroPro inhibitor, and mediates CARD8 inflammasome activation . In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (Caspase recruitment domain-containing protein 8, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the CARD8 inflammasome directly recruits pro-caspase-1 (proCASP1) independently of PYCARD/ASC and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis . Ability to sense HIV-1 protease activity leads to the clearance of latent HIV-1 in patient CD4(+) T-cells after viral reactivation; in contrast, HIV-1 can evade CARD8-sensing when its protease remains inactive in infected cells prior to viral budding . Also acts as a negative regulator of the NLRP3 inflammasome . May also act as an inhibitor of NF-kappa-B activation . .; FUNCTION: [Caspase recruitment domain-containing protein 8]: Constitutes the precursor of the CARD8 inflammasome, which mediates autoproteolytic processing within the FIIND domain to generate the N-terminal and C-terminal parts, which are associated non-covalently in absence of pathogens and other damage-associated signals. .; FUNCTION: [Caspase recruitment domain-containing protein 8, N-terminus]: Regulatory part that prevents formation of the CARD8 inflammasome: in absence of pathogens and other damage-associated signals, interacts with the C-terminal part of CARD8 (Caspase recruitment domain-containing protein 8, C-terminus), preventing activation of the CARD8 inflammasome . In response to pathogen-associated signals, this part is ubiquitinated by the N-end rule pathway and degraded by the proteasome, releasing the cleaved C-terminal part of the protein, which polymerizes and forms the CARD8 inflammasome (Probable) . .; FUNCTION: [Caspase recruitment domain-containing protein 8, C-terminus]: Constitutes the active part of the CARD8 inflammasome . In absence of pathogens and other damage-associated signals, interacts with the N-terminal part of CARD8 (Caspase recruitment domain-containing protein 8, N-terminus), preventing activation of the CARD8 inflammasome . In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing this form, which polymerizes to form the CARD8 inflammasome complex: the CARD8 inflammasome complex then directly recruits pro-caspase-1 (proCASP1) and promotes caspase-1 (CASP1) activation, leading to gasdermin-D (GSDMD) cleavage and subsequent pyroptosis . .

AccessionsE5RFI5
ENST00000447740.6 [Q9Y2G2-4]
ENST00000518622.5
ENST00000518979.5
ENST00000518450.5
ENST00000519332.5 [Q9Y2G2-6]
ENST00000651546.1 [Q9Y2G2-5]
ENST00000522773.5
ENST00000519940.6 [Q9Y2G2-5]
ENST00000517510.5
H0YB90
E5RIN1
ENST00000522431.5 [Q9Y2G2-6]
ENST00000521613.5 [Q9Y2G2-4]
B5KVR7
ENST00000391898.7 [Q9Y2G2-5]
E5RGG3
E5RJG3
ENST00000521415.5
ENST00000520753.5 [Q9Y2G2-3]
ENST00000522889.5
E5RGC9
E5RHZ3
ENST00000519646.5
I6L9C6
ENST00000520153.5 [Q9Y2G2-4]
E5RHJ3
ENST00000523579.5
H0YBW7
ENST00000521437.1
Q9Y2G2
ENST00000520007.5 [Q9Y2G2-6]
ENST00000519302.6
ENST00000520015.5 [Q9Y2G2-3]