RNF168 report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0016567Biological processprotein ubiquitination
GO_0006303Biological processdouble-strand break repair via nonhomologous end joining
GO_0036297Biological processinterstrand cross-link repair
GO_0010212Biological processresponse to ionizing radiation
GO_0045739Biological processpositive regulation of DNA repair
GO_0006302Biological processdouble-strand break repair
GO_0140861Biological processDNA repair-dependent chromatin remodeling
GO_0006974Biological processDNA damage response
GO_0006511Biological processubiquitin-dependent protein catabolic process
GO_0034244Biological processnegative regulation of transcription elongation by RNA polymerase II
GO_0097680Biological processdouble-strand break repair via classical nonhomologous end joining
GO_0070534Biological processprotein K63-linked ubiquitination
GO_0045190Biological processisotype switching
GO_0040029Biological processepigenetic regulation of gene expression
GO_0000151Cellular componentubiquitin ligase complex
GO_0032991Cellular componentprotein-containing complex
GO_0005654Cellular componentnucleoplasm
GO_0005829Cellular componentcytosol
GO_0035861Cellular componentsite of double-strand break
GO_0005634Cellular componentnucleus
GO_0070530Molecular functionK63-linked polyubiquitin modification-dependent protein binding
GO_0042393Molecular functionhistone binding
GO_0003682Molecular functionchromatin binding
GO_0140852Molecular functionhistone ubiquitin ligase activity
GO_0140858Molecular functionhistone H2AK15 ubiquitin ligase activity
GO_0046872Molecular functionmetal ion binding
GO_0031491Molecular functionnucleosome binding
GO_0004842Molecular functionubiquitin-protein transferase activity
GO_0005515Molecular functionprotein binding
GO_0043130Molecular functionubiquitin binding

IV. Literature review

[source]
Gene nameRNF168
Protein nameRING-type E3 ubiquitin transferase (EC 2.3.2.27)
E3 ubiquitin-protein ligase RNF168 (hRNF168) (EC 2.3.2.27) (RING finger protein 168) (RING-type E3 ubiquitin transferase RNF168)
Synonyms
DescriptionFUNCTION: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively). .

AccessionsENST00000437070.1
Q8IYW5
F8WD60
ENST00000318037.3