CHEK1 report

I. Expression across cell types

II. Expression across tissues

sc-RNAseq data

Insufficient scRNA-seq data for expression of CHEK1 at tissue level.

III. Associated gene sets

GO_0010569Biological processregulation of double-strand break repair via homologous recombination
GO_0045814Biological processnegative regulation of gene expression, epigenetic
GO_0006281Biological processDNA repair
GO_0045839Biological processnegative regulation of mitotic nuclear division
GO_0071260Biological processcellular response to mechanical stimulus
GO_0001833Biological processinner cell mass cell proliferation
GO_0006974Biological processDNA damage response
GO_0018107Biological processpeptidyl-threonine phosphorylation
GO_0090399Biological processreplicative senescence
GO_0070317Biological processnegative regulation of G0 to G1 transition
GO_0006260Biological processDNA replication
GO_0006338Biological processchromatin remodeling
GO_0007095Biological processmitotic G2 DNA damage checkpoint signaling
GO_0006997Biological processnucleus organization
GO_0044818Biological processmitotic G2/M transition checkpoint
GO_1901796Biological processregulation of signal transduction by p53 class mediator
GO_0046602Biological processregulation of mitotic centrosome separation
GO_1902742Biological processapoptotic process involved in development
GO_0006915Biological processapoptotic process
GO_0000086Biological processG2/M transition of mitotic cell cycle
GO_0071313Biological processcellular response to caffeine
GO_0006468Biological processprotein phosphorylation
GO_2000279Biological processnegative regulation of DNA biosynthetic process
GO_0042770Biological processsignal transduction in response to DNA damage
GO_0042127Biological processregulation of cell population proliferation
GO_0045787Biological processpositive regulation of cell cycle
GO_0000077Biological processDNA damage checkpoint signaling
GO_0005615Cellular componentextracellular space
GO_0005813Cellular componentcentrosome
GO_0005657Cellular componentreplication fork
GO_0032991Cellular componentprotein-containing complex
GO_0005654Cellular componentnucleoplasm
GO_0005829Cellular componentcytosol
GO_0005737Cellular componentcytoplasm
GO_0043231Cellular componentintracellular membrane-bounded organelle
GO_0000794Cellular componentcondensed nuclear chromosome
GO_0000781Cellular componentchromosome, telomeric region
GO_0000785Cellular componentchromatin
GO_0005634Cellular componentnucleus
GO_0019904Molecular functionprotein domain specific binding
GO_0106310Molecular functionprotein serine kinase activity
GO_0004674Molecular functionprotein serine/threonine kinase activity
GO_0004672Molecular functionprotein kinase activity
GO_0035402Molecular functionhistone H3T11 kinase activity
GO_0005524Molecular functionATP binding
GO_0005515Molecular functionprotein binding

IV. Literature review

[source]
Gene nameCHEK1
Protein nameSerine/threonine-protein kinase Chk1 (EC 2.7.11.1) (CHK1 checkpoint homolog) (Cell cycle checkpoint kinase) (Checkpoint kinase-1)
Serine/threonine-protein kinase Chk1 (EC 2.7.11.1) (CHK1 checkpoint homolog) (Checkpoint kinase-1)
Checkpoint kinase 1
non-specific serine/threonine protein kinase (EC 2.7.11.1)
CHEK1
SynonymsCHK1
DescriptionFUNCTION: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA . May also negatively regulate cell cycle progression during unperturbed cell cycles . This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome . Recognizes the substrate consensus sequence [R-X-X-S/T] . Binds to and phosphorylates CDC25A, CDC25B and CDC25C . Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C . Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A . Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A . Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression . Also phosphorylates NEK6 . Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination . Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation . Also promotes repair of DNA cross-links through phosphorylation of FANCE . Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A . This may enhance chromatin assembly both in the presence or absence of DNA damage . May also play a role in replication fork maintenance through regulation of PCNA . May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones (By similarity). Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes (By similarity). May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest . Phosphorylates SPRTN, promoting SPRTN recruitment to chromatin . Reduces replication stress and activates the G2/M checkpoint, by phosphorylating and inactivating PABIR1/FAM122A and promoting the serine/threonine-protein phosphatase 2A-mediated dephosphorylation and stabilization of WEE1 levels and activity . .; FUNCTION: [Isoform 2]: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition. .

AccessionsE7EPP6
ENST00000711049.1
ENST00000525396.5
ENST00000534070.5 [O14757-1]
ENST00000532449.6 [O14757-1]
E9PPA5
ENST00000527013.6
E9PKQ3
ENST00000428830.6 [O14757-1]
ENST00000526937.5
ENST00000532669.5
E9PQW7
E9PJI4
ENST00000427383.6
ENST00000534685.5
Q6W8P8
E9PRU7
ENST00000544373.5 [O14757-2]
A0A087WT52
ENST00000438015.7 [O14757-1]
ENST00000278916.8 [O14757-3]
ENST00000524737.6 [O14757-1]
ENST00000531607.5
O14757