DNA2 report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0032508Biological processDNA duplex unwinding
GO_0090656Biological processt-circle formation
GO_1902990Biological processmitotic telomere maintenance via semi-conservative replication
GO_0090304Biological processnucleic acid metabolic process
GO_0043504Biological processmitochondrial DNA repair
GO_0006264Biological processmitochondrial DNA replication
GO_0033567Biological processDNA replication, Okazaki fragment processing
GO_0006260Biological processDNA replication
GO_0071932Biological processreplication fork reversal
GO_0000729Biological processDNA double-strand break processing
GO_0044806Biological processG-quadruplex DNA unwinding
GO_0045740Biological processpositive regulation of DNA replication
GO_0032201Biological processtelomere maintenance via semi-conservative replication
GO_0000723Biological processtelomere maintenance
GO_0006284Biological processbase-excision repair
GO_0043137Biological processDNA replication, removal of RNA primer
GO_0000076Biological processDNA replication checkpoint signaling
GO_0042645Cellular componentmitochondrial nucleoid
GO_0005739Cellular componentmitochondrion
GO_0005760Cellular componentgamma DNA polymerase complex
GO_0005654Cellular componentnucleoplasm
GO_0005737Cellular componentcytoplasm
GO_0000781Cellular componentchromosome, telomeric region
GO_0005634Cellular componentnucleus
GO_0003677Molecular functionDNA binding
GO_0004386Molecular functionhelicase activity
GO_0016890Molecular functionsite-specific endodeoxyribonuclease activity, specific for altered base
GO_0017108Molecular function5'-flap endonuclease activity
GO_0003678Molecular functionDNA helicase activity
GO_0043139Molecular function5'-3' DNA helicase activity
GO_0004518Molecular functionnuclease activity
GO_0046872Molecular functionmetal ion binding
GO_0005524Molecular functionATP binding
GO_0003723Molecular functionRNA binding
GO_0017116Molecular functionsingle-stranded DNA helicase activity
GO_0005515Molecular functionprotein binding
GO_0051539Molecular function4 iron, 4 sulfur cluster binding
GO_0016887Molecular functionATP hydrolysis activity

IV. Literature review

[source]
Gene nameDNA2
Protein nameDNA replication ATP-dependent helicase/nuclease DNA2 (EC 3.6.4.12) (DNA replication ATP-dependent helicase-like homolog)
DNA replication ATP-dependent helicase/nuclease (EC 3.1.-.-) (EC 3.6.4.12)
DNA replication helicase/nuclease 2
DNA replication ATP-dependent helicase/nuclease DNA2 (hDNA2) (DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2 (EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2 (EC 3.6.4.12)]
SynonymsDNA2L
KIAA0083
DescriptionFUNCTION: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. .

FUNCTION: Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA. .

AccessionsENST00000399179.6 [P51530-2]
ENST00000551118.6
F8VR31
ENST00000440722.2
ENST00000358410.8 [P51530-1]
H0Y455
F8VPM6
ENST00000550357.1
P51530