WNK3 report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0140694Biological processnon-membrane-bounded organelle assembly
GO_0090279Biological processregulation of calcium ion import
GO_0072659Biological processprotein localization to plasma membrane
GO_0018107Biological processpeptidyl-threonine phosphorylation
GO_0006884Biological processcell volume homeostasis
GO_1903078Biological processpositive regulation of protein localization to plasma membrane
GO_1904062Biological processregulation of monoatomic cation transmembrane transport
GO_0035556Biological processintracellular signal transduction
GO_0043066Biological processnegative regulation of apoptotic process
GO_0010765Biological processpositive regulation of sodium ion transport
GO_2000651Biological processpositive regulation of sodium ion transmembrane transporter activity
GO_0035633Biological processmaintenance of blood-brain barrier
GO_0007231Biological processosmosensory signaling pathway
GO_0032414Biological processpositive regulation of ion transmembrane transporter activity
GO_0051928Biological processpositive regulation of calcium ion transport
GO_0071474Biological processcellular hyperosmotic response
GO_0090188Biological processnegative regulation of pancreatic juice secretion
GO_0050801Biological processmonoatomic ion homeostasis
GO_0006468Biological processprotein phosphorylation
GO_0010800Biological processpositive regulation of peptidyl-threonine phosphorylation
GO_0046777Biological processprotein autophosphorylation
GO_1903077Biological processnegative regulation of protein localization to plasma membrane
GO_0005912Cellular componentadherens junction
GO_0005737Cellular componentcytoplasm
GO_0005923Cellular componentbicellular tight junction
GO_0106310Molecular functionprotein serine kinase activity
GO_0004674Molecular functionprotein serine/threonine kinase activity
GO_0004672Molecular functionprotein kinase activity
GO_0140693Molecular functionmolecular condensate scaffold activity
GO_0005524Molecular functionATP binding
GO_0005515Molecular functionprotein binding

IV. Literature review

[source]
Gene nameWNK3
Protein nameSerine/threonine-protein kinase WNK3 (EC 2.7.11.1) (Protein kinase lysine-deficient 3) (Protein kinase with no lysine 3)
WNK lysine deficient protein kinase 3
SynonymsPRKWNK3
KIAA1566
DescriptionFUNCTION: Serine/threonine-protein kinase component of the WNK3-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress . WNK3 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation . The membraneless compartment concentrates WNK3 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK3-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK . Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A4/KCC1, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity . Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A4/KCC1, SLC12A5/KCC2 and SLC12A6/KCC3 inhibits its activity, blocking ion efflux . Phosphorylates WNK4, possibly regulating the activity of SLC12A3/NCC . May also phosphorylate NEDD4L . Also acts as a scaffold protein independently of its protein kinase activity: negatively regulates cell membrane localization of various transporters and channels, such as KCNJ1 and SLC26A9 . Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway . .

AccessionsENST00000354646.6 [Q9BYP7-1]
B1AQN8
ENST00000375159.6 [Q9BYP7-1]
Q9BYP7
ENST00000458404.1
ENST00000375169.7 [Q9BYP7-3]