SPRTN report

I. Expression across cell types

II. Expression across tissues

sc-RNAseq data

Insufficient scRNA-seq data for expression of SPRTN at tissue level.

III. Associated gene sets

GO_0036297Biological processinterstrand cross-link repair
GO_0016540Biological processprotein autoprocessing
GO_0006974Biological processDNA damage response
GO_0009411Biological processresponse to UV
GO_0106300Biological processprotein-DNA covalent cross-linking repair
GO_0006508Biological processproteolysis
GO_0031398Biological processpositive regulation of protein ubiquitination
GO_0019985Biological processtranslesion synthesis
GO_0016607Cellular componentnuclear speck
GO_0005654Cellular componentnucleoplasm
GO_0000785Cellular componentchromatin
GO_0005634Cellular componentnucleus
GO_0070530Molecular functionK63-linked polyubiquitin modification-dependent protein binding
GO_0003697Molecular functionsingle-stranded DNA binding
GO_0003690Molecular functiondouble-stranded DNA binding
GO_0046872Molecular functionmetal ion binding
GO_0004222Molecular functionmetalloendopeptidase activity
GO_0031593Molecular functionpolyubiquitin modification-dependent protein binding
GO_0005515Molecular functionprotein binding
GO_0043130Molecular functionubiquitin binding

IV. Literature review

[source]
Gene nameSPRTN
Protein nameDNA-dependent metalloprotease SPRTN (EC 3.4.24.-) (DNA damage protein targeting VCP) (DVC1) (Protein with SprT-like domain at the N terminus) (Spartan)
SprT-like N-terminal domain
SynonymsC1orf124
DVC1
UNQ1880/PRO4323
DescriptionFUNCTION: DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity . DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde . Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis . Catalyzes proteolytic cleavage of the HMCES DNA-protein cross-link following unfolding by the BRIP1/FANCJ helicase . Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4 . Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs (By similarity). SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs (By similarity). Involved in recruitment of VCP/p97 to sites of DNA damage . Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation . Does not activate CHEK1 in response to DNA damage . May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA . Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis . .

AccessionsQ9H040
ENST00000008440.9 [Q9H040-3]
ENST00000295050.12 [Q9H040-1]
ENST00000391858.8 [Q9H040-2]
B1AKT1
ENST00000366644.3