PRDM9 report

I. Expression across cell types

Insufficient scRNA-seq data for expression of PRDM9 at single-cell level.

II. Expression across tissues

sc-RNAseq data

Insufficient scRNA-seq data for expression of PRDM9 at tissue level.

III. Associated gene sets

GO_1905516Biological processpositive regulation of fertilization
GO_0010845Biological processpositive regulation of reciprocal meiotic recombination
GO_0010468Biological processregulation of gene expression
GO_0032259Biological processmethylation
GO_0007129Biological processhomologous chromosome pairing at meiosis
GO_0006338Biological processchromatin remodeling
GO_0048232Biological processmale gamete generation
GO_0043066Biological processnegative regulation of apoptotic process
GO_1990918Biological processdouble-strand break repair involved in meiotic recombination
GO_0006355Biological processregulation of DNA-templated transcription
GO_0006311Biological processmeiotic gene conversion
GO_0007292Biological processfemale gamete generation
GO_0005654Cellular componentnucleoplasm
GO_0005694Cellular componentchromosome
GO_0005634Cellular componentnucleus
GO_0042803Molecular functionprotein homodimerization activity
GO_0042800Molecular functionhistone H3K4 methyltransferase activity
GO_0140949Molecular functionhistone H3K9 trimethyltransferase activity
GO_0140955Molecular functionhistone H3K36 trimethyltransferase activity
GO_0000976Molecular functiontranscription cis-regulatory region binding
GO_0010844Molecular functionrecombination hotspot binding
GO_0140944Molecular functionhistone H4K20 monomethyltransferase activity
GO_0140941Molecular functionhistone H4K20me methyltransferase activity
GO_0008270Molecular functionzinc ion binding
GO_0140999Molecular functionhistone H3K4 trimethyltransferase activity
GO_0046975Molecular functionhistone H3K36 methyltransferase activity

IV. Literature review

[source]
Gene namePRDM9
Protein namePR/SET domain 9
PR domain zinc finger protein 9 (EC 2.1.1.43)
Histone-lysine N-methyltransferase PRDM9 (PR domain zinc finger protein 9) (PR domain-containing protein 9) (Protein-lysine N-methyltransferase PRDM9) (EC 2.1.1.-) ([histone H3]-lysine36 N-trimethyltransferase PRDM9) (EC 2.1.1.359) ([histone H3]-lysine4 N-trimethyltransferase PRDM9) (EC 2.1.1.354) ([histone H3]-lysine9 N-trimethyltransferase PRDM9) (EC 2.1.1.355) ([histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9) (EC 2.1.1.362) ([histone H4]-lysine20 N-methyltransferase PRDM9) (EC 2.1.1.361)
PR domain zinc finger protein 9
Histone methyl transferase (EC 2.1.1.43)
PRDM9
Histone-lysine N-methyltransferase PRDM9
PR-domain containing protein 9
SynonymsPFM6
DescriptionFUNCTION: Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination . Can also methylate all four core histones with H3 being the best substrate and the most highly modified . Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate (By similarity). During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity . Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression (By similarity). During meiotic progression hotspot-bound PRDM9 interacts with several complexes; in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity). In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation (By similarity). Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation (By similarity). In addition performs automethylation (By similarity). Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation (By similarity). .

FUNCTION: May be involved in transcriptional regulation. .

FUNCTION: May be involved in transcriptional regulation. .

AccessionsA0A1B3TN76
U5YR71
ENST00000502755.5
U5YUF5
ENST00000502755.6
D2E453
D2K7E6
A0A1B3TN77
D2K7E7
D2KI91
A0A1B3TN80
D2K7E9
D2E492
A0A1B3TN90
I7A6V7
ENST00000635252.1
I7AJB3
U5YS13
D2E456
A0A1D8X9D0
I7AFR5
A0A1B3TN79
I6V2Q9
A0A1B3TN81
A0A1B3TN96
A0A1B3TN72
U5YU24
D6RD68
D2KI84
I6ZLR0
U5YQZ9
I7A6W4
D2K7E5
I6ZAW9
D2KI88
D2KI89
ENST00000296682.4
A0A0U1RQY2
D2E482
D2KI86
U5YUF0
A0A1D8X9D4
D2E450
I6ZLR5
D2KI90
A0A1D8X9D6
Q9NQV7
D2K7E8
D2E459
D2K7F0
D2KI87
U5YS18
A0A1B3TN82
D2KI85
A0A1B3TN83
A0A1B3TN92