POLR3B report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_0051607Biological processdefense response to virus
GO_0032728Biological processpositive regulation of interferon-beta production
GO_0045089Biological processpositive regulation of innate immune response
GO_0042796Biological processsnRNA transcription by RNA polymerase III
GO_0045087Biological processinnate immune response
GO_0005654Cellular componentnucleoplasm
GO_0005829Cellular componentcytosol
GO_0005666Cellular componentRNA polymerase III complex
GO_0005737Cellular componentcytoplasm
GO_0003677Molecular functionDNA binding
GO_0071667Molecular functionDNA/RNA hybrid binding
GO_0001056Molecular functionRNA polymerase III activity
GO_0032549Molecular functionribonucleoside binding
GO_0008270Molecular functionzinc ion binding
GO_0003899Molecular functionDNA-directed 5'-3' RNA polymerase activity

IV. Literature review

[source]
Gene namePOLR3B
Protein nameDNA-directed RNA polymerase III subunit RPC2 (RNA polymerase III subunit C2) (EC 2.7.7.6) (C128) (DNA-directed RNA polymerase III 127.6 kDa polypeptide) (DNA-directed RNA polymerase III subunit B)
DNA-directed RNA polymerase (EC 2.7.7.6)
Synonyms
DescriptionFUNCTION: Catalytic core component of RNA polymerase III (Pol III), a DNA-dependent RNA polymerase which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Synthesizes 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci . Pol III-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol III is recruited to DNA promoters type I, II or III with the help of general transcription factors and other specific initiation factors. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA . Forms Pol III active center together with the largest subunit POLR3A/RPC1. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR3A/RPC1 contributing a Mg(2+)-coordinating DxDGD motif, and POLR3B/RPC2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate . Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway. .

AccessionsQ9NW08
F8VRU2
ENST00000228347.9 [Q9NW08-1]
ENST00000539066.5 [Q9NW08-2]
ENST00000549569.1