POLR1A report

I. Expression across cell types

II. Expression across tissues

III. Associated gene sets

GO_1904750Biological processnegative regulation of protein localization to nucleolus
GO_0006360Biological processtranscription by RNA polymerase I
GO_0005736Cellular componentRNA polymerase I complex
GO_0005694Cellular componentchromosome
GO_0005654Cellular componentnucleoplasm
GO_0003677Molecular functionDNA binding
GO_0071667Molecular functionDNA/RNA hybrid binding
GO_0001054Molecular functionRNA polymerase I activity
GO_0005515Molecular functionprotein binding
GO_0008270Molecular functionzinc ion binding
GO_0003682Molecular functionchromatin binding
GO_0000287Molecular functionmagnesium ion binding
GO_0003899Molecular functionDNA-directed 5'-3' RNA polymerase activity

IV. Literature review

[source]
Gene namePOLR1A
Protein nameRNA polymerase I subunit A
DNA-directed RNA polymerase subunit (EC 2.7.7.6)
DNA-directed RNA polymerase (EC 2.7.7.6)
DNA-directed RNA polymerase I subunit RPA1 (RNA polymerase I subunit A1) (EC 2.7.7.6) (A190) (DNA-directed RNA polymerase I largest subunit) (DNA-directed RNA polymerase I subunit A) (RNA polymerase I 194 kDa subunit) (RPA194)
Synonyms
DescriptionFUNCTION: Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs . Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Highly processive, assembles in structures referred to as 'Miller trees' where many elongating Pol I complexes queue and transcribe the same rDNA coding regions. At terminator sequences downstream of the rDNA gene, PTRF interacts with Pol I and halts Pol I transcription leading to the release of the RNA transcript and polymerase from the DNA . Forms Pol I active center together with the second largest subunit POLR1B/RPA2. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR1A/RPA1 contributing a Mg(2+)-coordinating DxDGD motif, and POLR1B/RPA2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and the template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate. Has proofreading activity: Pauses and backtracks to allow the cleavage of a missincorporated nucleotide via POLR1H/RPA12. High Pol I processivity is associated with decreased transcription fidelity (By similarity). .

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. .

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. .

AccessionsENST00000263857.11
Q96AG9
ENST00000409681.1
B7ZKR9
ENST00000409024.3
ENST00000424089.5
O95602
Q53T12
B9ZVN9
F2Z3I7