RAG2 report

I. Expression across cell types

II. Expression across tissues

sc-RNAseq data

Insufficient scRNA-seq data for expression of RAG2 at tissue level.

III. Associated gene sets

GO_0006325Biological processchromatin organization
GO_0002326Biological processB cell lineage commitment
GO_0035265Biological processorgan growth
GO_0002358Biological processB cell homeostatic proliferation
GO_0002313Biological processmature B cell differentiation involved in immune response
GO_1904155Biological processDN2 thymocyte differentiation
GO_0033151Biological processV(D)J recombination
GO_0033085Biological processnegative regulation of T cell differentiation in thymus
GO_0033077Biological processT cell differentiation in thymus
GO_0042742Biological processdefense response to bacterium
GO_0002360Biological processT cell lineage commitment
GO_0002331Biological processpre-B cell allelic exclusion
GO_0046622Biological processpositive regulation of organ growth
GO_0030183Biological processB cell differentiation
GO_0005654Cellular componentnucleoplasm
GO_0097519Cellular componentDNA recombinase complex
GO_0035064Molecular functionmethylated histone binding
GO_0061630Molecular functionubiquitin protein ligase activity
GO_0005547Molecular functionphosphatidylinositol-3,4,5-trisphosphate binding
GO_0043565Molecular functionsequence-specific DNA binding
GO_0003682Molecular functionchromatin binding
GO_0005546Molecular functionphosphatidylinositol-4,5-bisphosphate binding
GO_0035091Molecular functionphosphatidylinositol binding
GO_0008270Molecular functionzinc ion binding
GO_0080025Molecular functionphosphatidylinositol-3,5-bisphosphate binding
GO_0043325Molecular functionphosphatidylinositol-3,4-bisphosphate binding

IV. Literature review

[source]
Gene nameRAG2
Protein nameRecombination activating protein 2
Alternative protein RAG2
V(D)J recombination-activating protein 2 (RAG-2)
V(D)J recombination-activating protein 2
Synonyms
DescriptionFUNCTION: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity). .

AccessionsENST00000529083.1
E9PQB9
L0R5C5
P55895
ENST00000529083.2
Q9BYY1
ENST00000527033.6
E9PPU5
ENST00000532616.2
ENST00000311485.8
ENST00000527033.5