GEN1 report

I. Expression across cell types

II. Expression across tissues

sc-RNAseq data

Insufficient scRNA-seq data for expression of GEN1 at tissue level.

III. Associated gene sets

GO_0000724Biological processdouble-strand break repair via homologous recombination
GO_0010824Biological processregulation of centrosome duplication
GO_0031297Biological processreplication fork processing
GO_0071140Biological processresolution of mitotic recombination intermediates
GO_0090267Biological processpositive regulation of mitotic cell cycle spindle assembly checkpoint
GO_0071139Biological processresolution of DNA recombination intermediates
GO_0005813Cellular componentcentrosome
GO_0005654Cellular componentnucleoplasm
GO_0000400Molecular functionfour-way junction DNA binding
GO_0042803Molecular functionprotein homodimerization activity
GO_0008821Molecular functioncrossover junction DNA endonuclease activity
GO_0017108Molecular function5'-flap endonuclease activity
GO_0000287Molecular functionmagnesium ion binding

IV. Literature review

[source]
Gene nameGEN1
Protein nameGEN1 Holliday junction 5' flap endonuclease
Flap endonuclease GEN homolog 1 (EC 3.1.-.-)
Synonyms
DescriptionFUNCTION: Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation . Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction . Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA . Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates . .

AccessionsE9PLG0
Q17RS7
ENST00000532257.1
ENST00000524465.5
E9PM30
A0A087WXY3
ENST00000381254.7
ENST00000317402.11
ENST00000534451.2