A single-cell atlas of human and mouse white adipose tissue
Margo P. Emont, Christopher Jacobs, Adam L. Essene, Deepti Pant, Danielle Tenen, Georgia Colleluori, Angelica Di Vincenzo, Anja M. Jørgensen, Hesam Dashti, Adam Stefek, Elizabeth McGonagle, Sophie Strobel, Samantha Laber, Saaket Agrawal, Gregory P. Westcott, Amrita Kar, Molly L. Veregge, Anton Gulko, Harini Srinivasan, Zachary Kramer, Eleanna De Filippis, Erin Merkel, Jennifer Ducie, Christopher G. Boyd, William Gourash, Anita Courcoulas, Samuel J. Lin, Bernard T. Lee, Donald Morris, Adam Tobias, Amit V. Khera, Melina Claussnitzer, Tune H. Pers, Antonio Giordano, Orr Ashenberg, Aviv Regev, Linus T. Tsai, Evan D. Rosen
Abstract
White adipose tissue, once regarded as morphologically and functionally bland, is now recognized to be dynamic, plastic and heterogenous, and is involved in a wide array of biological processes including energy homeostasis, glucose and lipid handling, blood pressure control and host defence1. High-fat feeding and other metabolic stressors cause marked changes in adipose morphology, physiology and cellular composition1, and alterations in adiposity are associated with insulin resistance, dyslipidemia and type 2 diabetes2. Here we provide detailed cellular atlases of human and mouse subcutaneous and visceral white fat at single-cell resolution across a range of body weight. We identify subpopulations of adipocytes, adipose stem and progenitor cells, vascular and immune cells and demonstrate commonalities and differences across species and dietary conditions. We link specific cell types to increased risk of metabolic disease and provide an initial blueprint for a comprehensive set of interactions between individual cell types in the adipose niche in leanness and obesity. These data comprise an extensive resource for the exploration of genes, traits and cell types in the function of white adipose tissue across species, depots and nutritional conditions.
Datasets
1. A Single-Cell Atlas of Human White Adipose Tissue
Metadata
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Hs_SAT_SVF_242-2A-11751 cells Hs_SAT_SVF_236-1B-11433 cells Hs_SAT_SVF_235-2A-21416 cells Hs_SAT_SVF_238-1A-11369 cells Hs_SAT_SVF_236-1A-11317 cells Hs_SAT_SVF_236-1A-21248 cells Hs_SAT_SVF_239-2A-11218 cells Hs_SAT_SVF_235-2B-21201 cells Hs_SAT_SVF_235-2A-11180 cells Hs_SAT_SVF_238-1B-11148 cells Hs_SAT_SVF_235-1A-11129 cells Hs_SAT_SVF_242-2B-11116 cells Hs_SAT_SVF_235-2B-11109 cells Hs_SAT_SVF_239-1B-21098 cells Hs_SAT_SVF_238-2A-11040 cells Hs_SAT_SVF_238-2B-1998 cells Hs_SAT_SVF_236-2A-2990 cells Hs_SAT_SVF_239-3A-1796 cells Hs_SAT_SVF_237-1A-1705 cells Hs_SAT_SVF_239-1A-1671 cells Hs_SAT_SVF_235-1B-1670 cells Hs_SAT_SVF_237-2A-1647 cells Hs_SAT_SVF_235-1B-2548 cells Hs_SAT_SVF_239-3BC-1479 cells Hs_SAT_SVF_248-1A-1468 cells Hs_SAT_SVF_242-1B-1441 cells Hs_SAT_SVF_240-1B-1407 cells Hs_SAT_SVF_248-1B-1401 cells Hs_SAT_SVF_239-3B-1345 cells Hs_SAT_SVF_235-1A-2339 cells Hs_SAT_SVF_242-3A-1192 cells Hs_SAT_SVF_248-2A-1178 cells Hs_SAT_SVF_249-1A-1127 cells Hs_SAT_SVF_249-2B-1105 cells Hs_SAT_SVF_249-2A-197 cells Hs_SAT_SVF_249-1B-188 cells NCBITaxon:9606166149 cells UBERON:000219086064 cells UBERON:001041480085 cells HANCESTRO:0005120046 cells HANCESTRO:001425337 cells HANCESTRO:001620766 cells HsapDv:000013544037 cells HsapDv:000012318234 cells HsapDv:000013016416 cells HsapDv:000011815805 cells HsapDv:000012913316 cells HsapDv:000016212556 cells HsapDv:000016712038 cells HsapDv:000014710445 cells kilogram per square meter166149 cells mesothelial cell30482 cells endothelial cell15483 cells endothelial cell of lymphatic vessel2901 cells smooth muscle cell2886 cells immature NK T cell2629 cells stromal cell of endometrium312 cells subcutaneous adipose tissue86064 cells omental fat pad80085 cells Hispanic or Latin American25337 cells African American or Afro-Caribbean20766 cells 41-year-old human stage44037 cells 29-year-old human stage18234 cells 36-year-old human stage16416 cells 24-year-old human stage15805 cells 35-year-old human stage13316 cells 68-year-old human stage12556 cells 73-year-old human stage12038 cells 53-year-old human stage10445 cells 57-year-old human stage5711 cells 71-year-old human stage4607 cells 49-year-old human stage4555 cells 59-year-old human stage3907 cells 58-year-old human stage2066 cells 51-year-old human stage2039 cells 54-year-old human stage417 cells Analyze this study
Source data
https://cellxgene.cziscience.com/collections/fe0e718d-2ee9-42cc-894b-0b490f437dfd
Alias names
phs002766, SCP1376, GSE176171, GSE176067, PMID35296864, PMC9504827
Cite this study
Emont, M.P., Jacobs, C., Essene, A.L., Pant, D., Tenen, D., Colleluori, G., Di Vincenzo, A., Jørgensen, A.M., Dashti, H., Stefek, A. and McGonagle, E., 2022. A single-cell atlas of human and mouse white adipose tissue. Nature, 603(7903), pp.926-933. https://doi.org/10.1038/s41586-022-04518-2
