Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq

Nicole D Ulrich, Yu-chi Shen, Qianyi Ma, Kun Yang, D Ford Hannum, Andrea Jones, Jordan Machlin, John F Randolph Jr, Yolanda R Smith, Samantha B Schon, Ariella Shikanov, Erica E. Marsh, Jun Z Li, Sue Hammoud

Abstract

Fallopian tube (FT) homeostasis requires dynamic regulation of heterogeneous cell populations and is disrupted in infertility and ovarian cancer. Here, we applied single-cell RNA-seq to profile 59,738 FT cells from four healthy, pre-menopausal subjects. The resulting cell atlas contains 12 major cell types representing epithelial, stromal, and immune compartments. Re-clustering of epithelial cells identified four ciliated and six non-ciliated secretory epithelial subtypes, two of which represent potential progenitor pools: one leading to mature secretory cells and the other contributing to either ciliated cells or one of the stromal cell types. To understand how FT cell numbers and states change in a disease state, we analyzed 17,798 cells from two hydrosalpinx samples and observed shifts in epithelial and stromal populations and cell-type-specific changes in extracellular matrix and TGF-β signaling; this underscores fibrosis pathophysiology. This resource is expected to facilitate future studies aimed at expanding understanding of fallopian tube homeostasis in normal development and disease.

Datasets

1. scRNA-seq analysis of human fallopian tubes in healthy Vs. hydrosalphinx states
Metadata
donor_id
self_reported_ethnicity_ontology_term_id
donor_BMI
organism_ontology_term_id
sample_uuid
sample_preservation_method
tissue_ontology_term_id
development_stage_ontology_term_id
suspension_uuid
suspension_type
library_uuid
assay_ontology_term_id
mapped_reference_annotation
cell_type_ontology_term_id
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disease_ontology_term_id
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Dataset
tissue_type
cell_type
assay
disease
organism
sex
tissue
self_reported_ethnicity
development_stage
donor_225367 cells
donor_317354 cells
donor_515472 cells
donor_110457 cells
donor_46560 cells
donor_62326 cells
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Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq

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Dataset 1
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Source data

https://cellxgene.cziscience.com/collections/fc77d2ae-247d-44d7-aa24-3f4859254c2c

Alias names

GSE178101, PMID35320732, PMC9007916

Cite this study

Ulrich, N.D., Shen, Y.C., Ma, Q., Yang, K., Hannum, D.F., Jones, A., Machlin, J., Randolph, J.F., Smith, Y.R., Schon, S.B. and Shikanov, A., 2022. Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq. Developmental cell, 57(7), pp.914-929. https://doi.org/10.1016/j.devcel.2022.02.017